Efficacy of Low-Level Laser Therapy in Enhancing the Rate of Retraction by Assessment of β Glucoronidase and Pentraxin – 3 in Gingival Crevicular Fluid: A Clinical Study

Dr.Unnati Dinesh Parekh,*
Shraddha Shetti,
Jiwanasha Manish Agrawal,
Manish Agrawal,
Sangamesh Fulari

Abstract

INTRODUCTION: The study aimed to evaluate the efficacy of low-level laser therapy in enhancing the rate of orthodontic tooth movement using β glucuronidase and pentraxin-3 (TNF) biomarkers in the gingival crevicular fluid. METHOD: A split-mouth design was used in 16 subjects (8 experimental and 8 controlled) aged 14 to 25, whose maxillary first premolars were extracted. A gallium-aluminum-arsenide semiconductor diode laser (wavelength, 810 nm; energy density, 10 J/cm2; power output, 100 mW) delivered low-level laser therapy to the experimental canine undergoing distalization at 10 points. The control canine was destabilized without low-level laser therapy. The experimental and control canines were visualized using a force of 150 g provided by nickel-titanium closed coil springs. Gingival crevicular fluid was collected at 5-time points from the control and experimental sides, and the levels of β glucuronidase and pentraxin-3 were analyzed by enzyme-linked immunosorbent assay (ELISA). The rate of canine retraction was calculated by using ortho analyzer software. RESULT: Increased levels of β glucuronidase and pentraxin-3 were observed in the experimental canines compared with the control canines (P <0.001). Cumulative tooth movements over an 8-week experimental period were greater for the experimental canines compared with the control canines. A positive correlation existed between the β glucuronidase and pentraxin-3 levels and the amounts of tooth movement across all time intervals. CONCLUSION: Low-level laser therapy in adjunct with continuous and light forces leads to an increase in β glucuronidase and pentraxin-3 leading to accelerated orthodontic tooth movement.