Extraction of Hydroxyapatite Obtained From Human Premolar Teeth Using Smart Dentine Grinder and Calcination Process for Biomedical Application- An In vitro Study

• Author Details:   
• Hanish Anand ^*
• Gandikota Chandra Sekhar
• Ranjit Kumar Manne
• Nemani Shivaram
• Syed Shadab Husain

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Background: The present study is conducted with an aim to characterize Hydroxyapatite (HA) produced from therapeutically extracted premolar teeth using two different procedures. Hydroxyapatite was produced from premolar teeth using the process of calcination at 8500c and using Smart Dentine Grinder. The process of sample preparation influences its properties, the crystal structure and thermal stability of the produced powder was investigated using X-ray diffraction analysis (XRD) and Fourier transform infrared spectroscopic (FTIR) analysis. The former confirmed the successful production of HA crystals, while the latter confirmed the presence of hydroxyl (OH-) and phosphate (PO4-3) functional groups.
Results: The FTIR and XRD analysis graphs obtained for the samples from the two procedures were compared with graphs of Hydroxyapatite samples from previous studies.
Conclusion: FTIR and XRD analysis used to characterize the samples obtained from two procedures namely calcinations & using Smart Dentin Grinder confirmed the presence of Hydroxyapatite crystals. The procedure used has an effect on the crystalline properties of the Hydroxyapatite.

Keywords: Hydroxyapatite; Fourier transform infrared spectroscopic analysis; X-ray diffraction analysis (XRD); Calcination; Smart Dentine Grinder.